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Influence du polymorphisme du promoteur des sous-types du VIH-1 sur la dynamique de la réplication virale in vivo

Abstract : Viral promoters modulate transcription and hence viral replication. The HIV-1 promoter is polymorph in its transcription factors binding sites. The impact of this polymorphism on viral replication in vivo has never been addressed. On the background of a SIVmac239 genome, we have engineered three chimeras bearing the minimal promoters of HIV-1 clade B, C and E. By co-infection of four rhesus macaques, a discrepancy between the genotypes of rPBMCs-associated chimeras and of virions in the serum has been highlighted. A shift in viral genotype profiles was observed between the acute and the asymptomatic phase. These results show that subtype-specific HIV-1 promoter polymorphism is a major determinant influencing viral replication and dissemination. Notably, primary infection viremia is mainly due to HIV-1 replication in the gut.
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https://tel.archives-ouvertes.fr/tel-00010008
Contributor : Mireille Centlivre <>
Submitted on : Thursday, January 3, 2013 - 8:50:06 AM
Last modification on : Monday, October 19, 2020 - 10:53:24 AM
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  • HAL Id : tel-00010008, version 2

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Mireille Centlivre. Influence du polymorphisme du promoteur des sous-types du VIH-1 sur la dynamique de la réplication virale in vivo. Biochimie [q-bio.BM]. Université Pierre et Marie Curie - Paris VI, 2005. Français. ⟨tel-00010008v2⟩

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