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Development of an organ-on-chip microfluidic device incorporating an actuatable hydrogel layer to produce barrier tissue mimicries on chips

Abstract : Modern day ethics and laws call for more safety and use of fewer animals in biomedical research. It became crucial to develop novel in vitro devices of higher relevance. Since the end of the twentieth century, several systems have been proposed by researchers in attempts to palliate the shortcomings of current systems. Notably, organs-on-chip systems are specifically tailored to recapitulate tissue functions in a manner that remains easily accessible for the experimenter. Despite the significant improvements that were brought during the last century to in vitro cell and tissue culture systems, the field of bioengineering is still young and much progress remains to be done. The work presented here details the development of an organ-on-chip that includes a biocompatible and actuatable hydrogel membrane, with controlled physico-chemical properties. Such chip is relevant when hosting barrier tissues, which are composed of several cell types, disposed on each side of a barrier, as well as within its bulk, and are often submitted to mechanical stimuli. During this PhD, several objectives have been attained. Notably, we: - Designed and produced an organ-on-chip including a biocompatible and actuatable hydrogel layer, as well as a microfluidic system allowing the independent control of both flow and actuation. - Characterized the deformation of the hydrogel layer. - Cultured intestinal cells within the chip, which formed a three dimensionally structure epithelium, and characterized its apparent permeability to molecules of varying sizes
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Submitted on : Sunday, August 7, 2022 - 11:24:19 PM
Last modification on : Wednesday, September 14, 2022 - 10:24:10 AM

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  • HAL Id : tel-03747216, version 1

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Nicolas Minier. Development of an organ-on-chip microfluidic device incorporating an actuatable hydrogel layer to produce barrier tissue mimicries on chips. Bioengineering. Université de Technologie de Compiègne, 2021. English. ⟨NNT : 2021COMP2644⟩. ⟨tel-03747216⟩

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