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Modélisation in vitro des effets de toxiques environnementaux responsables de sécheresse oculaire, sur des cellules épithéliales et des neurones sensoriels trigéminés

Abstract : The ocular surface epithelia are at the forefront for protecting the eye from external aggressions, especially from the increasingly polluted and toxic environments that are implicated in the growing incidence of dry eye disease. The conjunctiva and even more, the cornea, innervated by the neurons of the trigeminal ganglia, respond to these aggressions by the establishment of inflammatory and nociceptive processes. Among the pollutants to which we are the most exposed are formaldehyde (FA) and benzalkonium chloride (BAK). We have tested in vitro on corneal and conjunctival epithelial cells and on trigeminal neurons, the toxic effects of these two xenobiotics. In a first study, we cultured in an air-liquid interface, WKD human conjunctival epithelial cells to expose them to a FA flow modeling a toxic stress. In a second study, we evaluated the interactions between epithelial cells and trigeminal neurons following toxic stress. Thus, a primary culture of trigeminal neurons was exposed to a conditioned medium produced by HCE human corneal epithelial cells previously exposed to BAK. In a third study, we developed a microfluidics compartmentalization model to culture trigeminal neurons, in order to study neuronal responses during BAK toxic stress only applied to the axonal endings. All together, our results highlight cellular and molecular mechanisms involved in some examples of ocular surface toxic stress.
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Submitted on : Tuesday, September 14, 2021 - 4:44:13 PM
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  • HAL Id : tel-03344179, version 1

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Michael-Adrien Vitoux. Modélisation in vitro des effets de toxiques environnementaux responsables de sécheresse oculaire, sur des cellules épithéliales et des neurones sensoriels trigéminés. Toxicologie. Sorbonne Université, 2020. Français. ⟨NNT : 2020SORUS165⟩. ⟨tel-03344179⟩

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