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Développement d’un pansement à libération contrôlée d’une protéine spécifique anti-biofilm bactérien. Application aux plaies chroniques.

Abstract : Bacterial biofilms are a major obstacle to the wound healing process. In addition, they are responsible for the emergence of resistance and tolerance to antibiotics. Hence, the development of controlled drug delivery systems targeting the bacterial biofilm appears as an urgent and essential alternative therapeutic approach for the effective management of chronic wound. In this work, we developed wound dressings in which a protein, dispersin B (DB), is released capable of selectively targeting the biofilm matrix, creating a deleterious microenvironment for the bacterial biofilm. To this end, we were interested in asymmetric membranes (AMs) from biodegradable polyesters such as the poly(3-hydroxybutyrate-co-4-hydroxybutyrate), the poly (butylene succinate-co-butylene adipate) (PBSA) and the polylactic acid. By the incorporation of hydrophilic porogen agents (PA), we were able to obtain AMs with a high level of porosity, exhibiting a porous interconnected network and oxygen and water vapor permeability. Using bovine serum albumin as a model protein, we demonstrated that protein loading and release from the PBSA AMs were affected by the membrane structure and the presence of residual PA. In vitro studies showed highest antibiofilm efficiency both in inhibition and dispersion (up to 80%). Normalized in vitro cytotoxicity standard assays revealed that unloaded and DB-loaded PBSA membranes met cytocompatibility criteria required for wound dressing applications.
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  • HAL Id : tel-02464378, version 2

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Naila Bou Haidar. Développement d’un pansement à libération contrôlée d’une protéine spécifique anti-biofilm bactérien. Application aux plaies chroniques.. Pharmacie galénique. Normandie Université, 2019. Français. ⟨NNT : 2019NORMR087⟩. ⟨tel-02464378v2⟩

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