Nouveaux rôles du régulateur de l'immunité MYB30 dans le développement d'Arabidopsis thaliana

Abstract : MYB30 is an Arabidopsis thaliana transcription factor known for its role of positive regulator of defense responses through the production of very long chain fatty acids (VLCFAs). The cuticle, a hydrophobic and protective layer that covers the aerial parts of the plant, is mainly composed of VLCFA-derived lipid molecules. In this thesis, we have shown that the MYB30 gene is expressed in the epidermal cells of the embryo, at a developmental stage that corresponds to the establishment of the embryonic cuticle. Our work reveals that during embryogenesis, MYB30 controls the expression of cuticle biosynthetic genes and the production of cuticular compounds. In agreement, we have shown that MYB30 is required for the formation of a functional embryonic cuticle. In addition, MYB30 expression is dependent on GASSHO1 and GASSHO2 (GSO1/2), two Receptor Like Kinases (RLKs) involved a seed-specific signaling pathway, necessary for the cuticle formation in the embryo. Our data also indicates that MYB30 negatively regulates GSO2 expression and that MYB30 may act downstream of the two RLKs in embryonic cuticle formation. Moreover, we have shown that these two RLKs also play a positive role in the activation of immune responses in adult plants. Finally, our study indicates that MYB30 is additionally involved in cell elongation in etiolated seedlings, likely by regulating the production of cell wall components. In conclusion, this work identifies new roles of MYB30, placing it at a crossroads between defense responses and plant development.
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Vincent Duplan. Nouveaux rôles du régulateur de l'immunité MYB30 dans le développement d'Arabidopsis thaliana. Biologie végétale. Université Paul Sabatier - Toulouse III, 2017. Français. ⟨NNT : 2017TOU30374⟩. ⟨tel-02454521⟩

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