Evaluation de la contribution d'une hémoglobine marine dans la culture cellulaire et dans la cellularisation de substituts osseux et méniscaux par des cellules souches mésenchymateuses

Abstract : This work aimed to develop cell culture systems, in 2D and 3D, based on the properties of HEMOXCell®, a marine oxygen carrier. Our approach was articulated in two main parts: the first one dealing with the assessment of the use of HEMOXCell® in the culture of two cellular models, and the second one, exploiting the results obtained for tissue engineering purposes. In this first axis, the dose-response effect of HEMOXCell® in the CHO-S cells and mesenchymal stem cells (MSC) in vitro culture, allowed the identification of optimal working concentrations, which can promote cell viability and proliferation. The CHO-S model has contributed to the establishment of a performance test of the molecule, and encouraged its use for bioproduction stimulation. The tests performed on MSCs were used to validate the harmlessness of the molecule at low doses and the maintenance of "stemness". The idea to associate MSCs with porous scaffolds is a promising approach for tissue engineering applications, but it is confronted to the lack of oxygen in the depth of the substitutes. In the second part of this project, we worked at improving the cellularization of bone and meniscal substitutes, under static and dynamic culture systems, w/ and w/o HEMOXCell®. In parallel, a study was conducted to attempt to characterize the meniscal cells. Analyses of cellularized biomaterials suggest a beneficial effect of HEMOXCell® when used as a differentiation media supplement. This work contributed to improve this oxygen carrier understanding and to extend the field of its potential uses particularly for therapeutic applications.
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Fiona Le Pape. Evaluation de la contribution d'une hémoglobine marine dans la culture cellulaire et dans la cellularisation de substituts osseux et méniscaux par des cellules souches mésenchymateuses. Biologie cellulaire. Université de Bretagne occidentale - Brest, 2016. Français. ⟨NNT : 2016BRES0002⟩. ⟨tel-02184573v2⟩

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