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Applications of time-resolved spectroscopy for microenvironment sensing and biomolecular interactions studies

Abstract : In the context of the present thesis, UV-Vis time-resolved spectroscopy was applied targeting the photophysics investigation of different environmentally sensitive molecular systems, but also as a biosensing approach to reveal molecular interactions or structural dynamics on much slower time scales (sec to min), using droplet microfluidics triggering structural relaxation through out-of-equilibrium initial conditions. I thus investigated on a three-axis-target spanning from 1) the in-depth investigation of the emissive properties of biosensors in order to allow their quantitative use in biomolecular interaction studies, to 2) the development of an original experimental approach to enable resolving the structural relaxation kinetics of an out-of-equilibrium distribution of biomolecular structures, and finally 3) the technological application of time resolved fluorescence for precise, rapid, cost effective, biomolecular interaction assays, appealing both for academic and industrial arenas.
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Submitted on : Wednesday, April 25, 2018 - 3:26:08 PM
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Anastasia Ioanna Skilitsi. Applications of time-resolved spectroscopy for microenvironment sensing and biomolecular interactions studies. Physics [physics]. Université de Strasbourg, 2017. English. ⟨NNT : 2017STRAE029⟩. ⟨tel-01777947v2⟩

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