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Étude de l'effet de la différention endothéliale sur les propriétés immunomodulatrices des cellules souches mésenchymateuses issues de la gelée de Wharton du cordon ombilical humain

Abstract : Umbilical cord Wharton’s jelly mesenchymal stem cells (WJ-MSC) are immune-privileged and immunosuppressive. Our study sought to determine the effect of endothelial differentiation on the immunomodulatory capacities of WJ-MSC. Endothelial-like cells (ECL) differentiation was performed by seeding MSC on polyelectrolyte multilayer films as substrate and stimulating them by EGM-2 culture medium. The expression of two immunological markers HLA-DR and CD86 was followed during the differentiation time. The effect of co-culture with ELC or MSC either in contact or separated by a transwell on different immune cells (peripheral blood mononuclear cells, T Lymphocytes (LT), Natural Killer (NK) cells) was assessed by evaluating immune cells proliferation. The results showed that ELC expressed endothelial markers, expressed low level of HLA-DR and CD86 and inhibited the proliferation of the different immune cell populations, and this inhibition is thought to be mediated by soluble factors as IDO, IL-6, IL-1[beta] and PGE2, and by direct cellular contact. We reported also the capacity of ELC to generate a population of regulatory T cells and to decrease the expression of activating receptor NKG2D by NK cells. Moreover, we demonstrated that co-cultures with ELC induce CD73 expression on NK cells, a mechanism that may induce adenosine (a potent immunosuppressor) secretion by NK cells. Thus, ELC maintain the hypo-immunogenic and immunosuppressive characters of WJ-MSC suggesting their possible use in vascular engineering
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https://hal.univ-lorraine.fr/tel-01750945
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Submitted on : Thursday, March 29, 2018 - 12:59:12 PM
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  • HAL Id : tel-01750945, version 1

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Reine El Omar. Étude de l'effet de la différention endothéliale sur les propriétés immunomodulatrices des cellules souches mésenchymateuses issues de la gelée de Wharton du cordon ombilical humain. Médecine humaine et pathologie. Université de Lorraine, 2014. Français. ⟨NNT : 2014LORR0126⟩. ⟨tel-01750945v1⟩

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