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Approche Microfluidique Polyvalente de la Cristallisation

Abstract : Crystallization is influenced by several parameters such as supersaturation, temperature, environment, and hydrodynamics. Thus, a rapid screening of crystallization conditions is often required during the study of the crystallization of a molecule. But when a small amount of material is available, a suitable experimental tool is essential. The development of microfluidic systems to form microdroplets has gained importance over the past decade (Leng & Salmon, 2009). Microfluidics brings together techniques that allow the manipulation of fluids on submillimeter scale. In our case, the microfluidic system dedicated to crystallization is based on the generation of nano-crystallizers (nanoliter droplets) isolated from each other and in which crystallization can occur independently. Forming hundreds of droplets which are monodisperse in size and experimental conditions, we can make a large number of experiments per condition for statistical studies to meet the stochasticity of the phenomenon of nucleation while consuming little material. Our goal is to create a universal microfluidic assembly which is compatible with all solvents and molecules. Thus, we are interested in simple microfluidic devices which allow generating droplets with PEEK junctions coupled with Teflon tubings which is resistant to many solvents (Ildefonso, Candoni, et al., 2012). From such systems, we have first characterized the hydrodynamic properties of the set-up: we sought to control microfluidic flow and reduce the volumes put-in and we studied the drop generation regimes by a "cross-flowing" method with two immiscible fluids in the T-junction. We present the scaling law of drop size in a systematic way which we relate to tangential shear. As part of the crystallization study of biomolecule, our goal is to screen different crystallization conditions while achieving a large number of experiments per condition for statistical analysis. We have validated our microfluidic set-up for crystallization studies in a viscous media, on the recombinant urate oxidase or the rasburicase in an aqueous solution of PEG. Finally, we integrated a module for on-line characterization of each droplet’s chemical composition.
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https://tel.archives-ouvertes.fr/tel-01230807
Contributor : Veesler Stephane <>
Submitted on : Tuesday, November 24, 2015 - 3:38:22 PM
Last modification on : Tuesday, March 30, 2021 - 3:21:43 AM
Long-term archiving on: : Friday, May 5, 2017 - 2:10:37 PM

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  • HAL Id : tel-01230807, version 1

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Shuheng Zhang. Approche Microfluidique Polyvalente de la Cristallisation. Physique [physics]. Aix Marseille Université, 2015. Français. ⟨tel-01230807⟩

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