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Développement d'un outil microfluidique polyvalent pour l'étude de la cristallisation : application à la nucléation de principes actifs pharmaceutiques

Manuel Ildefonso 1
1 SSP
CINaM - Centre Interdisciplinaire de Nanoscience de Marseille
Abstract : The aim of this work is to develop a microfluidic tool to study crystallization (and specifically nucleation) adapted to industrial issue, that's mean doing a lot of experiment with only few materials. Microfluidic, thanks to using nanoliters volume, are able to solve simultaneously both issues. Microfluidic system allows us to generate plenty of nanoliters droplets and each droplet is a microcristallizer to study nucleation. Here I present the development of a microfluidic system and the related analytical method dedicated to nucleation study of active pharmaceutical ingredient. As a first step we adapt an existing microfluidic system to study the nucleation of protein in water. Thanks to this system we are able to measure the metastable zone width and nucleation frequency of model protein used as an active pharmaceutical ingredient, the lysozyme. In a second step we modify this system in order to allow nucleation study in organic solvent. Thanks to this new system we can study the nucleations of different APIs using polyvalent methods develop to avoid nucleation problems due to the crystallization of API. This microfluidic system and the method develop to study nucleation of API are really polyvalent and let us imagine to extend their applicative field to all industrial field where using nanoliter volume can reduce the cost (protein crystallization) and/or risk (explosives, radioactive hazard).
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Submitted on : Friday, July 27, 2012 - 2:41:12 PM
Last modification on : Monday, January 27, 2020 - 11:20:25 AM
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Manuel Ildefonso. Développement d'un outil microfluidique polyvalent pour l'étude de la cristallisation : application à la nucléation de principes actifs pharmaceutiques. Science des matériaux [cond-mat.mtrl-sci]. Aix-Marseille université, 2012. Français. ⟨tel-00721436⟩

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