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Theses

Rôle de la protéine MtSNF4b dans la post maturation de graines de Medicago truncatula

Abstract : The sucrose nonfermenting-related kinase (SnRK1) together with its two regulatory subunits, b et g (SNF4), is an important regulator of plant metabolism and stress responses. The aim of this thesis is to study the role of MtSNF4b, a seed-specific g subunit in Medicago truncatula during imbibition and germination. A physiological analysis of seed germination of RNAi MtSNF4b lines during after-ripening demonstrates that seeds that do not express MtSNF4b show faster dormancy release. A comparative transcriptomic analysis between wild type and RNAi MtSNF4b seeds shows that most of the differentially expressed genes are repressed in the RNAi seeds and are linked to biotic stress. Genes code pathogenesis-related proteins (PR10), chitinases, WRKY transcription factors and enzymes involved in phenylpropanoïd and isoflavonoïd metabolic pathways leading to medicarpin biosynthesis. However, no difference in the sensitivity to Xanthomonas strains, based on dynamic analysis of the bacterial populations was detected between wild type or RNAi MtSNF4b seeds. Preliminary results of a double hybrid analysis reveal several putative candidates that might be implicated in the regulation of these genes. MtSNF4b appears to connect the expression of constitutive gene expression of defense responses to after-ripening in dormant seeds of M. truncatula.
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https://tel.archives-ouvertes.fr/tel-00459342
Contributor : Anne-Marie Plé <>
Submitted on : Tuesday, April 6, 2010 - 11:59:33 AM
Last modification on : Friday, October 23, 2020 - 4:35:33 PM
Long-term archiving on: : Thursday, September 23, 2010 - 12:37:35 PM

Identifiers

  • HAL Id : tel-00459342, version 2
  • PRODINRA : 248190

Citation

William Bolingue. Rôle de la protéine MtSNF4b dans la post maturation de graines de Medicago truncatula. Biologie végétale. Université d'Angers, 2009. Français. ⟨tel-00459342v2⟩

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