ORGANO-SPÉCIFICITÉ DE L'ENDOTHÉLIUM : MISE EN ÉVIDENCE ET CARACTÉRISATION D'UNE MOLÉCULE RÉGULATRICE DE L'ADHÉSION, NOMMEE ARM-1

Abstract : The LTP1 extracted from leaves of Nicotiana tabacum, named LTP1_1, was produced in Pichia pastoris. We have determined its three-dimensional structure by 2D and 3D NMR and molecular modelling. Various interaction and dynamic studies have highlighted the LTP1_1 particular binding properties compared to other LTPs. This work was used as a basis for the structure determination of others tobacco LTP1 using comparative modelling and for molecular dynamic simulations at ambient temperature and high temperature. Moreover, we mapped the LTP1_1 hydrophobic cavity by using hyperpolarized xenon NMR. This work allowed us to identify the amino acids involved specifically in lipid binding. We have discovered that the remarkable binding properties of LTP1 are related not only on their original structure but also in their particular dynamic.
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https://tel.archives-ouvertes.fr/tel-00009386
Contributor : Patricia Leloup <>
Submitted on : Monday, June 6, 2005 - 10:37:00 AM
Last modification on : Wednesday, June 27, 2018 - 8:24:02 AM
Long-term archiving on: Friday, April 2, 2010 - 10:11:14 PM

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  • HAL Id : tel-00009386, version 1

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Nathalie Lamerant-Fayel. ORGANO-SPÉCIFICITÉ DE L'ENDOTHÉLIUM : MISE EN ÉVIDENCE ET CARACTÉRISATION D'UNE MOLÉCULE RÉGULATRICE DE L'ADHÉSION, NOMMEE ARM-1. domain_other. Université d'Orléans, 2004. Français. ⟨tel-00009386v1⟩

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