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Theses

Bioinformatique des puces à ADN et application à l'analyse du transcriptome de Buchnera aphidicola

Abstract : The aim of this thesis is the study of the transcriptome of the bacterium Buchnera aphidicola, the primary endosymbiont of the pea aphid, Acyrthosiphon pisum, by microarray technology. The high interdependance between Buchnera and the aphid caused modifications of the bacterial genome, including loss of most regulatory genes. However, Buchnera retained the biosynthesis pathways, for the production of essential amino acids to its host. The first part of this thesis relates to the development of ROSO, a software to design optimized oligonucleotide probes for microarrays. An interface was conceived to allow its use on line (http://pbil.univ-lyon1.fr/roso). In the second part, the design and the use of a chip dedicated to Buchnera allowed to study the bacterial transcriptome, under nutritionnal and osmotic stress in the diet of the aphid. This analysis shows that Buchnera is able to modify its gene expression and to adapt its metabolism to answer to changing demand of its host.
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https://tel.archives-ouvertes.fr/tel-00008630
Contributor : Nancie Reymond <>
Submitted on : Wednesday, April 20, 2005 - 3:36:10 PM
Last modification on : Wednesday, July 8, 2020 - 12:42:09 PM
Long-term archiving on: : Thursday, September 23, 2010 - 3:52:53 PM

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  • HAL Id : tel-00008630, version 3
  • PRODINRA : 318762

Citation

Nancie Reymond. Bioinformatique des puces à ADN et application à l'analyse du transcriptome de Buchnera aphidicola. Sciences du Vivant [q-bio]. INSA de Lyon, 2004. Français. ⟨tel-00008630v3⟩

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