Skip to Main content Skip to Navigation
Journal articles

In vitro adhesion properties of shiga toxin-producing Escherichia coli isolated from cattle, food, and humans

Abstract : Shiga toxin-producing Escherichia coli (STEC) are able to cause serious illnesses ranging from diarrhea to hemorrhagic colitis and hemolytic-uremic syndrome (HUS). These bacteria colonize the digestive tract of humans and produce Shiga-toxins, which are considered to be essential for virulence and are crucial in lethal infection. Colon colonization is supposed to be a determinant step in the development of the infection, but the virulence traits that mediate this step are unclear. We analyzed the ability of 256 STEC strains belonging to seropathotype A (the most virulent 0157:H7 serotype) to seropathotype E (not involved in human disease) to adhere to HEp-2, HCT-8, and T84 cell lines. Of the 256 STEC tested most (82%) were non-adherent in our assays. The adhesion levels were globally low and were not related to pathogenicity, although the highest levels were associated to 026:H11 and 0103:H2 strains of seropathotype B (associated with HUS but less commonly than serotype 0157:H7), possessing both the eae and toxB genes.
Document type :
Journal articles
Complete list of metadatas

Cited literature [46 references]  Display  Hide  Download

https://hal.inrae.fr/hal-02636431
Contributor : Migration Prodinra <>
Submitted on : Wednesday, May 27, 2020 - 8:28:35 PM
Last modification on : Tuesday, February 23, 2021 - 11:40:02 AM

File

2015_Pradel_Frontiers in Micro...
Publisher files allowed on an open archive

Identifiers

Collections

Citation

Nathalie Pradel, Lucie Etienne Mesmin, Jonathan Thevenot, Charlotte Cordonnier, Stephanie Blanquet Diot, et al.. In vitro adhesion properties of shiga toxin-producing Escherichia coli isolated from cattle, food, and humans. Frontiers in Microbiology, Frontiers Media, 2015, 6, ⟨10.3389/fmicb.2015.00156⟩. ⟨hal-02636431⟩

Share

Metrics

Record views

40

Files downloads

61