Skip to Main content Skip to Navigation
New interface
Theses

Détection et pertinence clinique des Cellules Tumorales Circulantes (CTC) dans le Carcinome à Cellules de Merkel : Détermination du statut viral MCPyV des CTC vs biopsies tissulaires

Abstract : Merkel Cell Carcinoma (MCC) is a rare and aggressive skin cancer with an unfavorable prognosis related to early metastatic dissemination. The early detection is a major issue to improve the patient’s overall survival. Circulating Tumor Cells (CTCs) are cells that detached from solid tumor, go into the circulating system and can form distant metastasis. Their detection, by the liquid biopsy, can bring many clinical information, including early detection of cancer relapse.This thesis was aimed at detecting CTCs in Merkel Cell Carcinoma through the development of a new detection method in this malignancy. This R-D method (combining RosetteSep and DEPArray technologies) was compared to CellSearch, the Gold-Standard for CTC detection, in a cohort of 28 MCC patients. These two methods appeared complementary for CTC detection and the results were correlated with tumor stage. A second objective was to investigate the expression pattern of the Merkel cell Polyomavirus (MCPyV), involved in 80% of Merkel Cell Carcinoma overall, in single CTCs vs single cells isolated from tumor biopsies. This expression was heterogeneous in both sample subsets, a data not previously established. Finally, we tried to establish in-vitro cultures of CTCs isolated from patients’ blood samples but optimal conditions could not be set up to date.This work provides robust grounds for future clinical investigations carried out on larger cohorts of patients especially regarding the clinical relevancy of CTC detection as prognostic factor and outcome predictor in Merkel Cell Carcinoma, and for biological studies investigating MCPyV role in oncogenesis and systemic dissemination patho-mechanisms in this malignancy.
Document type :
Theses
Complete list of metadata

https://tel.archives-ouvertes.fr/tel-03655127
Contributor : ABES STAR :  Contact
Submitted on : Friday, April 29, 2022 - 12:10:22 PM
Last modification on : Friday, August 5, 2022 - 10:43:04 AM
Long-term archiving on: : Saturday, July 30, 2022 - 6:33:48 PM

File

2021_BOYER_archivage.pdf
Version validated by the jury (STAR)

Identifiers

  • HAL Id : tel-03655127, version 1

Collections

Citation

Magali Boyer. Détection et pertinence clinique des Cellules Tumorales Circulantes (CTC) dans le Carcinome à Cellules de Merkel : Détermination du statut viral MCPyV des CTC vs biopsies tissulaires. Médecine humaine et pathologie. Université Montpellier, 2021. Français. ⟨NNT : 2021MONTT069⟩. ⟨tel-03655127⟩

Share

Metrics

Record views

35

Files downloads

69