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Spatiotemporal control of Neural Stem Cell decisions in the adult zebrafish telencephalon

Abstract : In some regions of the adult vertebrate brain, Neural Stem Cells (NSCs) generate fully functional neurons. NSCs are found mostly in quiescence, but can shuttle from quiescence to activation (division). At the population level, the proportion of NSCs dividing at a given time remains constant throughout adulthood and perturbations of NSC activation rate correlate with pathological situations. Also, the spatiotemporal distribution of NSC activation events is expected to impact the homogeneous maintenance of the NSC pools and the locations of neuronal production. What controls the rate and spatiotemporal distribution of NSC activation events remain poorly understood. The adult zebrafish telencephalon is a good model to address these questions. The telencephalon hosts many NSCs and it allow the recording of their behaviors over weeks thanks to an intravital imaging procedure. In this thesis, we have used this model to study the regulation of adult NSCs behaviors from two perspectives. First, we assessed the existence of non-cell-autonomous mechanisms controlling the quiescence-activation balance of the NSC population in space and time. Second, we investigated the relevance of intrinsic heterogeneities on individual NSC behaviors. This work highlighted (i) the importance of NSC geometry for their fate decisions during activation and (ii) the role of their differentiated progeny to locally exert a delayed inhibition, via Notch signaling, to prevent neighboring NSC activation. Using modeling we also show how the lineage-related inhibition maintains NSCs with specific spatiotemporal correlations and can spatially homogenize the distribution of adult-born neurons.
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https://tel.archives-ouvertes.fr/tel-03342474
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Submitted on : Monday, September 13, 2021 - 1:09:41 PM
Last modification on : Wednesday, September 15, 2021 - 5:43:30 PM

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MANCINI_Laure_2020_v2.pdf
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  • HAL Id : tel-03342474, version 1

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Laure Mancini. Spatiotemporal control of Neural Stem Cell decisions in the adult zebrafish telencephalon. Development Biology. Sorbonne Université, 2020. English. ⟨NNT : 2020SORUS154⟩. ⟨tel-03342474⟩

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