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Development of genetic control strategies for insect pests using CRISPR/Cas9

Abstract : Insect pest control remains an important economic, environmental, and public health challenge. CRISPR/Cas9 gene drive (GD) is a novel genetic control strategy. GDs are genetic systems that can rapidly invade a population. This manuscript presents my efforts to develop gene drives in two important pest species, Anopheles gambiae, a major vector of malaria, and Drosophila suzukii, a global crop pest. The goals of this project were to develop a suppression gene drive in D. suzukii, to reduce population size, and a modification drive in An. gambiae, to reduce malaria transmission. While I was unable to produce a functional gene drive in D. suzukii, the efforts and protocols presented here can serve as a baseline for future work in this economically important crop pest. In An. gambiae, I successfully characterized two transgenic lines, one of which significantly blocks malaria transmission to a rodent model. Finally, I present my efforts to engineer a new modification gene drive strategy, indirect gene drive.
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  • HAL Id : tel-03336532, version 1

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Emily Green. Development of genetic control strategies for insect pests using CRISPR/Cas9. Animal genetics. Université de Strasbourg, 2019. English. ⟨NNT : 2019STRAJ100⟩. ⟨tel-03336532⟩

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