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Theses

Le transport intraflagellaire : construction et déplacement des trains dans le flagelle du trypanosome

Abstract : Cilia and flagella are essential organelles composed of 9 doublet microtubules. They contain at least 500 proteins and their construction is mainly done by adding new subunits at the distal end. They are transported byIntraflagellar transport (IFT), the movement of trains composedof two protein complexes between the flagellar membrane and the microtubule doublets by driven by molecular kinesin and dynein motors. My thesis project is based on the role and functioning of IFT using the protistTrypanosoma brucei as a model organism. The goal of my thesis project was (i) to determine how IFT trains are assembled by establishing the link between their molecular composition and their structure and (ii) to establish the route taken by IFT trains within the flagella. By combining light microscopy and electron microscopy approaches after RNAi targeting of genes coding for IFT train components, we have demonstrated their contribution to the construction of IFT trains. We propose a new model to explain train formation and their entry in the flagellum. By three-dimensional electron microscopy (FIB-SEM), we have also shown where IFT trains are located. Trains are specifically found on 4 microtubule doublets out of the 9 available. These results have been obtained bothin vitro and ex vivousing parasites developing in the tsetse fly.Comparison of the results with the literature highlights the flexibility of transport depending on the anatomy of cilia and flagella.
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Submitted on : Wednesday, April 7, 2021 - 1:05:11 PM
Last modification on : Friday, April 9, 2021 - 3:32:03 AM

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  • HAL Id : tel-03191671, version 1

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Adeline Antunes. Le transport intraflagellaire : construction et déplacement des trains dans le flagelle du trypanosome. Biologie cellulaire. Sorbonne Université, 2020. Français. ⟨NNT : 2020SORUS080⟩. ⟨tel-03191671⟩

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