Skip to Main content Skip to Navigation
Theses

Mise au point de méthodes de recherche large de xénobiotiques par chromatographie liquide couplée à la spectrométrie de masse en tandem

Abstract : Screening methods for compounds of interest in clinical toxicology have been historically employed as first-line analysis to either confirm or exclude the hypothesis of drug overdose, or poisoning with a toxicant. After identification using a screening method, quantification using specific methods is often necessary in a second time to assess the severity of intoxication. This obviously increases the turnaround time and has an impact on patient care. With regards to the rapidity needed to report results in clinical toxicology, sample extraction may be a limiting step. There is no universal extraction procedure for human samples prior to screening analysis Our goal were to developed screening procedures using a programmable liquid handler (CLAM-2000; Shimadzu®) directly coupled to a liquid chromatography - tandem mass spectrometry (LC-MS 8060; Shimadzu®).Libraries with spectral and chromatographic parameters were built for about 1300 compounds selected based on their relevance to, and occurrence in, clinical toxicology. Using these libraries, screening methods were developed using different acquisition modes.Two different extraction procedures were developed: one using QuEChERS salts, another using the CLAM-2000.Each approach was validated according to the ISO 15189 requirements and was applied to real patient samples. The different screening methods yielded high confidence in compound detection and should be useful in core labs facing clinical toxicology situations where rapid and reliable results are needed.
Document type :
Theses
Complete list of metadata

https://tel.archives-ouvertes.fr/tel-03163213
Contributor : Abes Star :  Contact
Submitted on : Tuesday, March 9, 2021 - 10:32:13 AM
Last modification on : Thursday, March 11, 2021 - 5:01:24 PM
Long-term archiving on: : Thursday, June 10, 2021 - 6:28:30 PM

File

2020LIMO0052.pdf
Version validated by the jury (STAR)

Identifiers

  • HAL Id : tel-03163213, version 1

Collections

Citation

Tiphaine Robin. Mise au point de méthodes de recherche large de xénobiotiques par chromatographie liquide couplée à la spectrométrie de masse en tandem. Médecine humaine et pathologie. Université de Limoges, 2020. Français. ⟨NNT : 2020LIMO0052⟩. ⟨tel-03163213⟩

Share

Metrics

Record views

59

Files downloads

161