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Development of a hybrid coating associating biomimetic hydroxyapatite and natural antibiotics with antibacterial properties

Abstract : This thesis aims to develop a hybrid coating based on biomimetic hydroxyapatite and natural antibacterial molecules, with the goal to add antibacterial properties and enhance the osseointegration of titanium (Ti6Al4V) implants. Four natural biologically active molecules (BAMs) were tested, rosmarinic acid, chlorogenic acid, baicalin and baicalein. To understand the interaction between those molecules and hydroxyapatite, we present here three complementary studies: in solution with calcium ion, with hydroxyapatite nanoparticles and with biomimetic hydroxyapatite coatings on titanium alloy. The complexation between BAMs and calcium ion in solution is highly pH-dependent. When incorporated within hydroxyapatite nanoparticles or coatings during synthesis, BAMs inhibit the formation of the mineral phase. On the contrary, they can be efficiently adsorbed on pre-synthesized particles. Finally, we present the biological properties of hydroxyapatite nanoparticles and coatings with adsorbed baicalein. Hybrid nanoparticles are potent antioxidant agents, but they do not exhibit any antibacterial activity, whereas hybrid coatings have a significant effect on the bacterial growth of Staphylococcus epidermidis CIP 105.777. This work originality stands on the use of natural antibacterial molecules, but we highlight the difficulty to handle them, due to their poor solubility and stability in physiological pH, which should be considered for further use in biomedical applications.
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https://tel.archives-ouvertes.fr/tel-03141223
Contributor : Abes Star :  Contact
Submitted on : Monday, February 15, 2021 - 10:34:37 AM
Last modification on : Tuesday, February 16, 2021 - 3:30:02 AM

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PALIERSE_Estelle_2019.pdf
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  • HAL Id : tel-03141223, version 1

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Estelle Palierse. Development of a hybrid coating associating biomimetic hydroxyapatite and natural antibiotics with antibacterial properties. Material chemistry. Sorbonne Université, 2019. English. ⟨NNT : 2019SORUS316⟩. ⟨tel-03141223⟩

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