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Microstructuration de surface et protection par encapsulation : applications aux biocapteurs

Abstract : This thesis explores two ways to enhance the performance of an electrochemical affinity biosensor for the detection of dengue anti-NS1 antibodies.The first goal was to improve the limit of detection and sensitivity of biosensors using microstructuration of the transducer. Based on finite element modelling, an optimal range of the microcone geometric parameters was defined. After construction, the microcone-covered electrodes were characterised using cyclic voltammetry and scanning electron microscopy. At the end of the optimisation process, glucose biosensors were fabricated to demonstrate the increased performance gains of an amperometric biosensing platform. Finally, in order to validate the interest of the microcones for an affinity biosensor, a label-free impedimetric immunosensor allowing the quantification of the cholera toxin B-subunit was studied as a model before performing an immunosensor of interest for dengue antibodies. The immunosensors were made on two types of electrodes: microcone-coated electrodes and interdigitated electrodes.A second goal was to improve the shelf-life of lateral-flow biosensors by microencapsulating labelled biomolecules, mandatory for competitive detection. For this purpose, low-cost polymer microcapsules have been developed. Methylene blue and an enzyme, glucose oxidase, were encapsulated in a dry state. Their release was followed by UV-visible spectroscopy and/or electrochemistry after breaking the microcapsules by mechanical action and/or with ultrasonic agitation.
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Submitted on : Friday, November 20, 2020 - 11:57:33 AM
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Marie Berthuel. Microstructuration de surface et protection par encapsulation : applications aux biocapteurs. Chimie-Physique [physics.chem-ph]. Université Grenoble Alpes [2020-..], 2020. Français. ⟨NNT : 2020GRALV002⟩. ⟨tel-03016182⟩

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