Skip to Main content Skip to Navigation
Theses

Caractérisation et modélisation de la culture de la microalgue Chlamydomonas reinhardtii et mise en place d'un photobioréacteur miniaturisé pour l'étude quantitative de la croissance de micro-organismes photosynthétiques

Abstract : Funded by two separate research projects (AlgoH2 (ANR) and MELiSSA (ESA)), this work, conducted within the GePEB axis, is focused on the application of knowledge models of energetics of living cells for cultures of photosynthetic microorganisms. The first study was conducted on the microalga Chlamydomonas reinhardtii, in order to optimize the bio- production of dihydrogen. The work has been to gather the whole metabolic reactions existing in the cell in order to carry out a stoichiometric and thermokinetic study of the cellular functioning of growth and hydrogen production phases, the objective being to understand, control and optimize the production of hydrogen in photobioreactor. A contribution of this work is also to assemble a consistent database, bringing together the properties of formation of compounds involved in the cellular functioning, in order to apply the rules of calculations of thermochemical equilibria to compounds of cellular metabolism. As a second part of the study, a mini-photobioreactor developed in the lab in 2003 has been optimized and modernized. This mini-PBR allows realizing fast analyzes on the functioning of the photosynthesis. The design in two compartments separated by a membrane presents the interest extrapolating the behavior of the cyanobacterium Arthrospira platensis in conditions of microgravity. A model of knowledge of the photosynthesis, grouping the radiative transfer and the kinetics of growth on nitrate source had previously been developed. Comparing the preliminary tests carried out in Roux flasks on different nutrient nitrogen sources with model allowed better understanding of the cellular metabolism and further improvements of the mini-PBR.
Complete list of metadatas

Cited literature [191 references]  Display  Hide  Download

https://tel.archives-ouvertes.fr/tel-02978417
Contributor : Abes Star :  Contact
Submitted on : Monday, October 26, 2020 - 2:12:07 PM
Last modification on : Wednesday, October 28, 2020 - 3:07:24 AM

File

2017CLFAS023.pdf
Version validated by the jury (STAR)

Identifiers

  • HAL Id : tel-02978417, version 1

Citation

Nastasia Soanen. Caractérisation et modélisation de la culture de la microalgue Chlamydomonas reinhardtii et mise en place d'un photobioréacteur miniaturisé pour l'étude quantitative de la croissance de micro-organismes photosynthétiques. Sciences agricoles. Université Clermont Auvergne, 2017. Français. ⟨NNT : 2017CLFAS023⟩. ⟨tel-02978417⟩

Share

Metrics

Record views

82

Files downloads

104