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Planar polarization of a mono-ciliated epithelium : the zebrafish floor-plate

Abstract : Planar cell polarization (PCP) of motile cilia allows directional fluid flow generation within body cavities. However the mechanisms underlying PCP of ciliated epithelia are still poorly understood. Here we use the zebrafish floor-plate (FP) to investigate these mechanisms. Using both fixed samples and live-imaging, we show that asymmetric cilia positioning at the posterior side of FP cells is a progressive and asynchronous process between neighbouring cells. As development proceeds, basal bodies (BB, modified centrioles at the base of cilia) spend more time in contact with the posterior membrane and stop making contacts with the anterior membranes. We investigate the role of Par3 in FP cells polarization. We find that Par3 is enriched at the posterior membrane, before the asymmetric positioning of cilia. Strikingly, at early stages, Par3 accumulates as patches at the level of apical junctions and BB only contact anterior or posterior membranes at the level of these patches. In addition, Par3 over-expression disrupts FP PCP. These results strongly suggest that Par3 is a critical player in FP polarization. Finally we show that in zebrafish vangl2 PCP mutants, Par3 patches intensity and polarisation are disrupted, which could explain FP polarization defects. Since Vangl2 is anteriorly enriched in FP cells, this suggests an antagonistic relationship between Par3 and Vangl2.
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Submitted on : Wednesday, October 21, 2020 - 10:39:42 AM
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  • HAL Id : tel-02973647, version 1

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Antoine Donati. Planar polarization of a mono-ciliated epithelium : the zebrafish floor-plate. Development Biology. Sorbonne Université, 2019. English. ⟨NNT : 2019SORUS078⟩. ⟨tel-02973647⟩

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