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, ) based on their morphological features (FSC/SSC). Data acquisition and analysis were performed with the BD PlateManager and BD CellQuestPro software, BD Biosciences
, After 10 days, cells were washed with PBS, fixed with 70% ethanol, washed again and then stained with a 1% Crystal Violet solution (Sigma-Aldrich, St. Quentin Fallavier, France). of 5000 cells were seeded into 96-well plates in six replicates. The day after, the culture medium was replaced by a doxorubicin-containing medium (0.2 mL per well), Clonogenic Assay Totals of 100, 500, and 1000 cells were plated onto 6-well plates
TMRM) A total of 5000 cells were seeded into 96-well plates in at least three replicates. The day after, the culture medium was replaced by drug-containing medium (0.2 mL per well), Mitochondrial Potential Loss Assay, vol.20 ,
5% CO 2 for 30 min. Then, supernatants were collected, cells were trypsinized and resuspended in their respective supernatants, TMRM-staining was analyzed by flow cytometry (FACS Calibur, BD Biosciences ,
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