Skip to Main content Skip to Navigation
Theses

Rôle de la protéine CASK dans l'organisation des sous populations de canaux ioniques dans le myocyte et dans l'électrophysiologie cardiaque in vivo

Abstract : Cardiomyocytes are highly differentiated cells with well-organized membrane domains containing distinct ion channels with specific roles in electrical and mechanical cardiac properties. However, the functional expression of ion channels in these membrane domains is regulated by complex processes that are just starting to be identified. The multidomain proteins of the MAGUK (Membrane Associated GUanylate Kinase) family are implicated in trafficking, anchoring and organization of macromolecular complexes at the plasma membrane. For the first time, we have identified in the heart a member of these scaffolding proteins named CASK (Calcium/Calmodulin-dependant Serine protein Kinase). CASK has a restricted localization at the lateral membrane where it binds to dystrophin-glycoprotein complex through its HOOK domain and a subpopulation of cardiac sodium channel NaV1.5. Moreover, CASK is able to negatively regulate the sodium current INa in the cardiac myocyte by impeding anterograde trafficking and/or retention of NaV1.5 channels in submembrane compartments through its L27B or GUK domains. By maintaining low levels of sodium channels at the lateral membrane of cardiac cells, CASK could participate in maintenance of the anisotropic conduction of the depolarization wave in the myocardium.
Complete list of metadatas

Cited literature [60 references]  Display  Hide  Download

https://tel.archives-ouvertes.fr/tel-02947049
Contributor : Abes Star :  Contact
Submitted on : Wednesday, September 23, 2020 - 4:06:34 PM
Last modification on : Wednesday, October 14, 2020 - 4:08:54 AM

File

BEURIOT_Adeline_2018.pdf
Version validated by the jury (STAR)

Identifiers

  • HAL Id : tel-02947049, version 1

Citation

Adeline Beuriot. Rôle de la protéine CASK dans l'organisation des sous populations de canaux ioniques dans le myocyte et dans l'électrophysiologie cardiaque in vivo. Biochimie, Biologie Moléculaire. Sorbonne Université, 2018. Français. ⟨NNT : 2018SORUS528⟩. ⟨tel-02947049⟩

Share

Metrics

Record views

57

Files downloads

12