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Control of meiotic divisions in oocytes : a novel role for cyclin B3

Abstract : Meiosis is a tightly regulated process made up of two successive divisions, meiosis I and II. They must be completed in an orderly manner to obtain haploid gametes with the correct number of chromosomes. Female mammalian meiosis is an error-prone process where errors in segregation create aneuploid gametes. In addition, incidence of aneuploidy increases in correlation with age. Understanding the regulation of female mammalian meiosis is therefore essential. Meiotic cell divisions are regulated by cyclins associated to their binding catalytic partners Cdks. I investigated the role of a unique cyclin, cyclin B3, through the use of cyclin B3 KO female mice. I found that lack of cyclin B3-Cdk1 activity in KO oocytes affects APC/C activity and induces an arrest at metaphase I due to high cyclin B1 levels, high Cdk1 activity, and inactive separase. Surprisingly, cyclin B3 from other species was able to rescue mouse cyclin B3 KO oocytes. I was also able to show that cyclin B3 is able to inhibit CSF arrest. My recent data suggests that cyclin B3 KO oocytes put in place a precocious CSF arrest, leading to the metaphase I arrest observed. Hence, my PhD work has shown that cyclin B3 is essential for female meiosis I and to prevent precocious CSF arrest in meiosis I instead of meiosis II.
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Nora Bouftas. Control of meiotic divisions in oocytes : a novel role for cyclin B3. Subcellular Processes [q-bio.SC]. Sorbonne Université, 2019. English. ⟨NNT : 2019SORUS176⟩. ⟨tel-02932167⟩

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