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. La-protéine-de-la-nucléocapside, En utilisant les analogues nucléosidiques fluorescents thienoguanosine et 2-thiényl-3-hydroxychromone, nous avons déterminé l'ensemble des constantes de vitesse cinétiques du mécanisme d'hybridation de la séquence (-)PBS avec (+)PBS en absence et en présence de NC. Nous avons également étudié le rôle du NA sucre dans les complexes NC-ARN et NC-ADN, puisque la protéine NC se lie avec la polarité opposée aux séquences d'ADN et d'ARN. Nous avons confirmé que l, NC) possède un rôle important dans le cycle de viral du VIH-1 grâce à sa propriété chaperone des acides nucléiques (NA) qui implique la reconnaissance de son résidu Trp37 avec un résidu Guanine de l'acide nucléique cible

. Enfin, nous avons réalisé un criblage de potentiels inhibiteurs de la protéine NC et examiné les touches identifiées à partir d'un test basé sur la fluorescence de la sonde th G

, Using the fluorescent thienoguanosine ( th G) and 2-thienyl-3-hydroxychromone (3HCnt) nucleoside analogues, we determined the whole set of kinetic rate constants for annealing of (-)PBS with (+)PBS in the absence and presence of NC. We also investigated the role of NA sugar in NC-RNA and NC-DNA complexes, as NC binds with opposite polarity to DNA and RNA sequences. We confirmed that the interaction of the Trp37 residue with guanines was critical for the formation of complexes with both RNA and DNA variants of PBS and SL3. Finally, Mots-clés : VIH-1, nucléocapside protéine, analogues fluorescents, thiéoguanosine, PBS, SL3, acides nucléiques dynamique, fluorescence

, Key words: HIV-1, nucleocapsid protein, fluorescent nucleobase analogues, thieoguanosine, primer binding site, SL3, nucleic acid dynamics, fluorescence spectroscopy, protein-nucleic acids interaction