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Study of the interactome of UPF1, a key factor of Nonsense-mediated decay in Arabidopsis thaliana

Abstract : The RNA helicase UPF1 is a key factor of Nonsense-Mediated Decay (NMD), a paneukaryotic mechanism involved in mRNA quality control and fine-tuning of gene expression. Despite important biological functions in plants, NMD is poorly described compared to other eukaryotes. This thesis presents the identification and study of UPF1 interacting proteins in Arabidopsis. Using approaches based on immunoaffinity and mass spectrometry, we identified a novel protein-protein interaction network between UPF1 and translation repressors in P-bodies. We propose a model in which translation repression exerts a protective action on NMD targets in plants. Our approach also identified novel P-body components, including the UCN endonuclease. A detailed study revealed its direct link with the decapping machinery and possible roles in hormone signaling and defense mechanisms, suggesting that the modulation of UCN expression could influence important agronomical traits. This work describes hitherto unknown UPF1 associated factors, their study will provide novel insights into the mechanisms involved in the balance between mRNA translation, storage and decay in plants.
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Clara Chicois. Study of the interactome of UPF1, a key factor of Nonsense-mediated decay in Arabidopsis thaliana. Molecular biology. Université de Strasbourg, 2018. English. ⟨NNT : 2018STRAJ005⟩. ⟨tel-02918112⟩

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