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Structure de l'ARN au sein des ribonucléoprotéines des Influenzavirus A

Abstract : The Influenzavirus genome comprises eight segments of single-stranded RNA of negative polarity packaged in viral ribonucleoproteins (vRNP). Genome segmentation complicates packaging as a full set of vRNPs is required needed for virus infectivity. It is now accepted that packaging is selective and involves interactions between the RNA components of vRNPs that likely depend on the RNA structure. Our goal was to characterize the binding of the viral protein NP, the major component of the vRNP, on in vitro transcribed RNA. We also developed an experimental strategy to determine the RNA structure inside the viral particles. Both parts of the project were addressed with chemical mapping experiments, that interrogates the flexibility of each nucleotide in the RNA structure. Our results show that NP possess an RNA chaperone activity and binds preferential sites. Our in viro approach demonstrate that using two chemical probes allow us to discriminate between RNA-RNA and RNA-NP interactions.
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Damien Ferhadian. Structure de l'ARN au sein des ribonucléoprotéines des Influenzavirus A. Virologie. Université de Strasbourg, 2018. Français. ⟨NNT : 2018STRAJ066⟩. ⟨tel-02918074⟩

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