Skip to Main content Skip to Navigation
Theses

Study of the role of the human TREX-2 complex in the DNA Damage Response

Abstract : The maintenance of proper genetic information is essential to avoid genomic instability, which is a hallmark of cancer. In response to Double Strand Breaks (DSBs), cells initiate the DNA Damage Response (DDR), that acts through two main sub-pathways: non-homologous end joining (NHEJ) and homologous recombination (HR). The nuclear pore-associated TREX-2 complex is involved in mRNA export and has been implicated, in yeast, in genome stability maintenance. Here we investigated the role of TREX-2 in DSB repair in human cells. We find that loss of the scaffold subunit of TREX-2 (GANP) results in DNA repair deficiency by HR. Moreover, we showed that the mechanism through which TREX-2 protects human cells from DNA damage is dependent on an interplay with the co-activator complex SAGA that regulates H2Bub1 histone mark. Our results demonstrate a functional cross-talk between human TREX-2 and the SAGA deubiquitination activity that is important to ensure correct DSB repair during HR.
Complete list of metadatas

Cited literature [30 references]  Display  Hide  Download

https://tel.archives-ouvertes.fr/tel-02918065
Contributor : Abes Star :  Contact
Submitted on : Thursday, August 20, 2020 - 10:18:03 AM
Last modification on : Monday, September 21, 2020 - 12:03:07 PM

File

EVANGELISTA_Federica_2017_ED41...
Version validated by the jury (STAR)

Identifiers

  • HAL Id : tel-02918065, version 1

Collections

Citation

Federica Evangelista. Study of the role of the human TREX-2 complex in the DNA Damage Response. Genomics [q-bio.GN]. Université de Strasbourg, 2017. English. ⟨NNT : 2017STRAJ122⟩. ⟨tel-02918065⟩

Share

Metrics

Record views

24

Files downloads

21