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Structural rearrangements of the HIV-1 genomic RNA during maturation of the viral particle

Abstract : The HIV-1 particle buds from the infected cell as an immature particle and has to undergo a maturation process to become infectious. Proteolytic processing of Pr55Gag triggers morphological rearrangements of the particle whereas the gRNA dimer becomes more stable. Genomic rearrangements remain poorly understood and are facilitated by the RNA chaperone activity of the NCp7 protein. Our goal was to determining the different steps leading to the formation of the mature dimeric gRNA. To this end, the structure of the first 550 nucleotides of the HIV-1 genome was assessed by chemical probing 1. in vitro with Pr55Gag, GagΔp6, NC-containing intermediates and NCp7 proteins and 2. in viro with the hSHAPE-Seq approach we developed. Wild type and mutant viruses mimicking the sequential processing of Pr55Gag were analysed, as well as immature PR- and mature particles treated with the AT-2 zinc ejector, in order to identify the Pr55Gag and NCp7 binding sites and their gRNA destabilising activity.
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Submitted on : Thursday, August 20, 2020 - 9:53:11 AM
Last modification on : Friday, August 21, 2020 - 3:29:08 AM


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  • HAL Id : tel-02917974, version 1



Élodie Mailler. Structural rearrangements of the HIV-1 genomic RNA during maturation of the viral particle. Virology. Université de Strasbourg, 2017. English. ⟨NNT : 2017STRAJ055⟩. ⟨tel-02917974⟩



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