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Apport de l'antibiofilmogramme et de la mesure de la capacité de formation du biofilm dans la prise en charge des infections ostéo-articulaires à staphylocoques

Abstract : In the context of Bone and Joint Infections (BJIs), the orthopedic devices are preferential surface for microorganisms to adhere and form biofilm associated with high rates of failures and relapses. Within biofilm, bacteria are protected from the host immune response and are able to survive in the presence of high concentration of antibiotics. The standard Minimal Inhibitory Concentration (MIC) informs on the antibiotic susceptibility of planktonic bacteria, but is not suited for biofilm. The company BioFilm Control developed a new test named Antibiofilmogram® which measures early-stage biofilm growth in presence of antibiotics, and provides a biofilm Minimal Inhibitory Concentration (bMIC). The aim of my PhD research was first to take part in the demonstration of the clinical value of this new test for Staphylococcus aureus BJIs. We established a prospective collection of data and strains and realized the first in vitro assays. Our results for cloxacillin were confirmed in an in vivo model of catheter-associated infection. Second, we characterized the biofilm formation capacity of various clinical isolates based on the Antibiofilmogram® resistance profile. We showed that the biofilm formation capacity is correlated with clonal lineage. Finally, we were able to develop a new method of washing and quantifying biofilms for microplate system using steam. This simple approach preserves the biofilm integrity and lead to highly reproducible data
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https://tel.archives-ouvertes.fr/tel-02917955
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Submitted on : Thursday, August 20, 2020 - 9:48:11 AM
Last modification on : Tuesday, August 25, 2020 - 4:54:43 PM

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  • HAL Id : tel-02917955, version 1

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Jason Tasse. Apport de l'antibiofilmogramme et de la mesure de la capacité de formation du biofilm dans la prise en charge des infections ostéo-articulaires à staphylocoques. Microbiologie et Parasitologie. Université de Lyon, 2017. Français. ⟨NNT : 2017LYSE1115⟩. ⟨tel-02917955⟩

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