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Theses

Développement d'un pansement intelligent par détection de metalloprotéases

Abstract : In 2017, it was estimated that 425 million people worldwide had diabetes. The main reason for this skyrocketing number is related to the current socio-economic context of the world with global expenses reaching 1.3 billion dollars a year with estimates reaching up to 2.1 billion dollars by 2030 (Bommer et al., Diabetes Care, 2018). Above all physiological unbalances, this disease can cause many side effects if left untreated, one of them being chronic wounds, wounds that don’t follow the usual healing process. Among the different types of chronic wounds is diabetic foot ulcers (DFUs), with a prevalence of 6.3% (Sen, Advances in Wound Care, 2019) and a lifetime risk for the diabetic population as high as 25% (Boulton et al., The Lancet, 2005). These wounds have many factors involved in the permanent inflammation state that they are in, one of them being matrix metalloproteinases (MMPs) zinc endopeptidases with various roles.This project is focused on the development of a smart dressing for the monitoring of DFUs. This monitoring is done by measuring the concentration of a key enzyme in the inflammation process, MMP9 or Gelatinase B. This system is based on the use of porous alumina functionalized with a Biological Recognition Element (BRE) able to detect MMP9.The first part of the project is the search for BREs based of the screening of camelid antibodies (VHHs) or peptide libraries by phage display. Then, the project focuses on the development of two prototypes of capacitive biosensors, one for the study of the functionalization of a conventional anti-MMP9 antibody, the other with the use of a third-party VHH and its target, GFP. These two proofs-of-concept will be useful for the continuity of the project.
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Pauline Choma. Développement d'un pansement intelligent par détection de metalloprotéases. Ingénierie de l'environnement. IMT - MINES ALES - IMT - Mines Alès Ecole Mines - Télécom, 2019. Français. ⟨NNT : 2019EMAL0010⟩. ⟨tel-02893629⟩

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