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Hydrogels thermosensibles et mucoadhésifs : nouvelles stratégies pour prévenir et traiter les pathogènes au niveau de la muqueuse vaginale

Abstract : According to the latest WHO estimates, 498 millions of new cases of sexually transmitted infections (STIs) are recorded annually in the world, including 276.4 million due to the parasite Trichomonas vaginalis (T. vaginalis). In the genital tract colonization and irritation of the vaginal mucosa by T. vaginalis promote the occurrence of infectious complications. Associated infections can lead to chronic infections and eventually have serious consequences (infertility, premature placental abruption, premature death). In addition, these vaginal infections can promote infection by Human Immunodeficiency Virus (HIV-1).Currently, the fight against these infections is to act at curative and preventive level. Thus, the objective of this project is to develop new formulations for the prevention and treatment of pathogens that colonize the vaginal mucosa. In this context, we propose a formulation composed of metronidazole and chitosan include in a thermogelling hydrogel of pluronic F127®.It was shown that the hydrogel retains its physical properties even at a physiological temperature and after dilution in the vaginal fluids. These hydrogels are stable and allow a sustained release of the metronidazole. The formulation showed no toxicity against HeLa cells or porcine vaginal mucosa. The effectiveness of this formulation has been proven against T vaginalis and has a protective effect on HeLa cells in the presence of T. vaginalis. The overall results therefore suggest the ability of this formulation to form a double barrier, physical and pharmacological, than protect vaginal mucosa against T. vaginalis.
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Bénédicte Pradines. Hydrogels thermosensibles et mucoadhésifs : nouvelles stratégies pour prévenir et traiter les pathogènes au niveau de la muqueuse vaginale. Médecine humaine et pathologie. Université Paris Sud - Paris XI, 2014. Français. ⟨NNT : 2014PA114813⟩. ⟨tel-02889749⟩

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