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Résumé L'eutrophisation des cours d'eau du monde entier favorise le developpement des Cyanobactéries. Certaines espèces sont potentiellement productrices de métabolites secondaires. Cependant si certains métabolites sont des toxines pouvant être dangereux pour l'homme, d'autres par contre peuvent être d'un, GSC Biological and Pharmaceutical Sciences, vol.8, issue.1, pp.84-88, 2019. ,
, Enfin la PCR conventionnelle a permis de détecter les gènes qui codent pour la synthèse des microcystines, cyanopeptolines, microviridines et aeruginosines. Le suivi des paramètres physico-chimiques de la lagune Aghien a permis de mettre en évidence une évolution relativement importante de la conductivité et des sels nutritifs (phosphore et phosphate). Les teneurs en chlorophylle varient de 28,26 ?g/L à 95,05 ?g/L avec des valeurs maximales obtenues pendant les saisons sèches, L'objectif général de ce travail a été d'identifier les Cyanobactéries de la lagune Aghien et d'étudier leur potentiel à produire des métabolites secondaires
, Les genres Pseudanabaena et Aphanothece ont été les moins mis en évidence (59,09%), Ils sont suivis des genres Aphanocapsa (95,45%), Planktothrix (90,90%), p.1682
, 74%) Cyanobactéries ont eu une croissance positive sur ce milieu. Les gènes de microcystines mcyA et mcyD ont été détectés respectivement dans 93,75% et 62,5% des échantillons. Les autres métabolites secondaires : aeruginosines (aerD), cyanopeptolines (mcnC) et microviridines (mdnB) ont été obtenues respectivement dans 87,5%, 50% et 18,75% des échantillons. En plus des gènes de synthèse des microcystines, les Cyanobactéries de la lagune Aghien possèderaient d'autres gènes de métabolites secondaires, Cyanobactéries ont été isolées et purifiées par des repiquages successifs sur la gélose BG11, 563 (33,47%) ont été obtenues. Les 563 Cyanobactéries ont été transférées sur milieu BG11 liquide, vol.38