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Biomarqueurs de l'arthrose : analyse tribologique et microspectroscopie Raman du liquide synovial

Abstract : Osteoarthritis is the most common joint disorder worldwide. Symptomatic osteoarthritis is a painful and debilitating disease which is associated with a large physical, psychological, societal and economic burden. According to the United Nations, by 2050, 315 million people will suffer from symptomatic osteoarthritis worldwide, of whom 105 million will be severely disabled by the disease.Osteoarthritis has traditionally been diagnosed and followed-up based on clinical symptoms and imaging modalities. However, these techniques and molecular biomarker developments are insensitive and identification of patients at risk to disease progression remains a challenge. They also fail to subgroup the heterogenous osteoarthritis population while this would allow a better scrutiny of diagnosis and treatment options. A surrogate biomarker with prognostic and/or diagnostic capabilities able to identify potential responders is much needed in osteoarthritis care and therapy.In diarthrodial joints, homeostasis is based on physiological composition and function of synovial fluid, a viscous fluid that lubricates joints. During osteoarthritis, synovial fluid biochemical properties are modified simultaneously to other joint tissues damages.In this work, a minimally invasive technique has been developed to characterize synovial fluid osteoarthritic state in order to diagnose, predict and follow-up joint diseases in patients and animals. Deposition of only a few microliters of synovial fluid on fused silica slide forms a circular dried drop with a ring-like deposit of concentrated solutes at the droplet edge. Tribological analysis based on the morphology of the dried drops reflects synovial fluid biomolecular and structural modifications. Raman Spectroscopy provides more specific information on chemical composition and monitors biomolecular structural alterations. This thesis leads to the proposal of synovial biomarkers representative of the joint osteoarthritic process.
Keywords : Osteoarthritis
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Catherine Bosser. Biomarqueurs de l'arthrose : analyse tribologique et microspectroscopie Raman du liquide synovial. Autre. Université de Lyon, 2017. Français. ⟨NNT : 2017LYSEC046⟩. ⟨tel-02881448⟩

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