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Theses

Développements en chimie bioorthogonale pour des applications en protéomique chimique et en pharmacocinétique

Abstract : The main goal of this work was to synthesize chemical tools and to developp their biological applications. In the first part, probes for the study of Topoisomerase II via chemical proteomic were synthesized. They were then used for pulldown experiments on cell lysats. In a second part, a new cleavable linker in non denaturing conditions was developped for chemical proteomic applications. After optimisation of the structure, it was incorporated in an affinity probe and tested for the pulldown of PARP 1. Finally, a click chemistry reaction, the SPAAC, was used to provok the elimination of a circulating drug. After the study of the kinetic of the reaction, the biological activity and the pharmacokinetic of the different compounds were evaluated to optimise the click reaction in vivo.
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Marion Recher. Développements en chimie bioorthogonale pour des applications en protéomique chimique et en pharmacocinétique. Sciences pharmaceutiques. Université de Strasbourg, 2014. Français. ⟨NNT : 2014STRAF025⟩. ⟨tel-02872895⟩

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