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Hétérocycles fluorescents pour la détection des protéines carbonylées associées au vieillissement et à l’inflammation

Abstract : The works presented in this manuscript describe the synthesis of new fluorescent probes for protein labelling. In a first part, the synthesis of new fluorescent molecules 1,2,4-triazoles based 1,3,5-trisubstituted is described from 4H-pyrido[e][1,3]oxazin-4-ones. The photophysical study of 1,2,4-triazoles in several solvents allowed to highlight a fluorescence ESIPT type mechanism leadingto a wide Stokes’shift (close to 250 nm). The second part of this manuscript is dedicated to the synthesis of new BODIPY with a hydrazide function. Finally, the last part describes the applications of these new BODIPY as fluorescent probes for carbonylated proteins labelling, used in 2D - Oxi DIGE, fluorescence microscopy and flow cytometry.
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Anthony Nina Diogo. Hétérocycles fluorescents pour la détection des protéines carbonylées associées au vieillissement et à l’inflammation. Chimie organique. Sorbonne Université, 2018. Français. ⟨NNT : 2018SORUS355⟩. ⟨tel-02865500⟩

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