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La Trypanosoma cruzi proline racemase, un marqueur diagnostique et de suivi pour la Maladie de Chagas ?

Abstract : Chagas disease caused by Trypanosoma cruzi occurs in Latin and Central America. The parasite is difficult to detect outside the acute phase of infection. No diagnostic means is 100% reliable and does not allow detection of the parasite in the tissues and no vaccine is available. The laboratory characterized the first eukaryotic proline racemase which is also a mitogen for B cells: Trypanosoma cruzi proline racemase (TcPRAC). It is a virulence factor for the parasite, essential for its survival. TcPRAC genes are present in all genotypes of the parasite and are absent in other infectious trypanosomatids. TcPRAC could be a specific marker for the disease. We have demonstrated, using the experimental model, that TcPRAC is indeed a specific diagnostic marker of the infection but especially a marker that would make it possible to assert the effectiveness of a treatment. We were able to follow the infectious process in real time by in vivo imaging. We have produced anti-TcPRAC VHHs capable of binding to intracellular TcPRAC expressed by live parasites in vitro. We have initiated the development of a prototype for the detection of intracellular parasites in vivo. We have been able to optimize new trypanocidal compounds in order to develop new chemotherapies against the disease.
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Nicolas Coatnoan. La Trypanosoma cruzi proline racemase, un marqueur diagnostique et de suivi pour la Maladie de Chagas ?. Microbiologie et Parasitologie. Sorbonne Université, 2018. Français. ⟨NNT : 2018SORUS315⟩. ⟨tel-02864792⟩

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