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Procédé d'encapsulation à base d'hydrogels pour le développement de micro-tissus cellulaires

Abstract : This thesis concerns the improvement of a hydrogel encapsulation process for three-dimensional cell culture. Submillimetric capsules are formed via high speed co-extrusion of macromolecules solutions, thereby forming a compound jet. The drops resulting from the fragmentation of the jet have a core/ shell type geometry where shell is composed of alginate. This layer is then solidified after immersion in a gelling bath. The use of biological materials required the implementation of a sequential injection system to manipulate small volumes, less than 1 mL. This approach is then accompanied by a longitudinal variation of the concentration of the suspended particles flowing in the injection tube. This dispersion can be inhibited by adding air bubbles at each end of the sample to segment it. A destabilization of the suspension initially homogeneous is observed when liquid inertia comes into play at the particle’s scale. These micrometric particles induce a flapping motion and jet speed fluctuations causing the coalescence of the drops and thus a size polydispersity. Finally, a collagen hydrogel, which mimics an extracellular matrix, has been implemented in the capsule’s core to promote adhesion of epithelial cells forming intestine and bile ducts. Within this matrix, the cells form a polarized and functional epithelium. The formation of these collagen capsules required the formulaiton of the collagen solution compatible with the process and the physiological conditions of the cells.
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  • HAL Id : tel-02862449, version 1

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Wafa Bouhlel. Procédé d'encapsulation à base d'hydrogels pour le développement de micro-tissus cellulaires. Mécanique des fluides [physics.class-ph]. Sorbonne Université, 2018. Français. ⟨NNT : 2018SORUS414⟩. ⟨tel-02862449⟩

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