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Système microfluidique pour le contrôle et l'optimisation de l'encapsulation de cellules pour la thérapie du diabète

Abstract : Transplantation of microcapsules containing pancreatic islets, cell clusters regulating blood sugar, show promising results for type 1 diabetes therapy. However, many challenges remain to improve long-term graft functionality. The lack of standardization of current encapsulation technologies has aroused interest in microfluidic systems that enable more precision and automation.This thesis focuses on two of the current encapsulation technologies stakes: improving system productivity and microcapsules surface.In the first part of this thesis, we characterized a pressure-driven microfluidic flow focusing device (MFFD) droplet generation system. Analytical and numerical models were developed in order to determine and predict flow rates. Droplet formation was characterized as a function of the system input parameters. This study led to scaling laws enabling to predict these system input parameters in order to optimize alginate microcapsules production frequency.In the second part of this thesis, a microfluidic system enabling the production of core-shell microcapsules was developed. First experiments of pancreatic islets encapsulation have shown the ability of this system to minimize the immune reaction towards these capsules.This work is a first step towards encapsulation system optimization, which eventually, may provide capsules that meet all the capsule requirements for transplantation.
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  • HAL Id : tel-02628493, version 1

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Claire Authesserre. Système microfluidique pour le contrôle et l'optimisation de l'encapsulation de cellules pour la thérapie du diabète. Alimentation et Nutrition. Université Grenoble Alpes, 2016. Français. ⟨NNT : 2016GREAS017⟩. ⟨tel-02628493⟩

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