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Développement des outils d'imagerie par résonance magnétique nécessaires à l'étude du chien, du cheval et du mouton en neuroimagerie

Abstract : More and more frequently, preclinical studies on animal models use MRI acquisitions. However, the acquisition of images from an animal brain has some differences with that conducted in humans, which therefore requires adaptations of the sequences. Once the acquisition is completed, the second problem concerns the tools and methodology available for image post-processing. Indeed, these tools all exist for humans (Template, Atlas, Standardization tools and segmentation), but few animal models have them. Thus, literature and methodological resources are very poor with regard to the Dog, yet increasingly used as a model of study in neuroscience. In this study, we mainly focused on the implementation of these tools in the Beagle. For this, 23 laboratory dogs without neurological disorders were anesthetized to make acquisitions in a 3 Tesla MRI. The acquisition protocol consisted of T1 and T2 weighting, and DTI. For the first time, T1, T2 and DTI weighted templates both in-vivo and post-mortem have been implemented. In addition, the DTI Templates allow a tractography and thus several sets of fibers could be reconstructed (Cingulum, Corpus Callosum, Fornix, Internal Capsule and Rostral Commissure). In addition to the MRI indices, histological sections were taken from a Beagle brain from the study cohort. Two stains were performed: one for myelin and the other for Nissl's substance. The histological sections were correlated with the different MRI Templates. The principle is to allow a user to have the correspondence of a Template voxel or one of these normalized acquisitions with histological sections. In addition, this study has also enabled the implementation of various methodological tools that have been adapted to the study of other animal species: Cat, Sheep and Horse.
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Submitted on : Tuesday, May 26, 2020 - 6:12:07 PM
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Samuel Boucher. Développement des outils d'imagerie par résonance magnétique nécessaires à l'étude du chien, du cheval et du mouton en neuroimagerie. Imagerie médicale. Université Paul Sabatier - Toulouse III, 2019. Français. ⟨NNT : 2019TOU30187⟩. ⟨tel-02626836⟩

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