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Tomographie de diffraction sans mesure de phase en régime de diffusion multiple

Kevin Unger 1, 2
2 SEMO - SEMO
FRESNEL - Institut FRESNEL
Abstract : Optical diffraction tomography is an imaging technique in which a target is successively illuminated by a laser beam under different angles of incidence with an interferometric setup which enables to record both the phase and the intensity of the field in the image space of a microscope. The permittivity of the target is then numerically estimated from the complex field dataset thanks to an inversion procedure. In this typescript, we first study the effect of multiple scattering inside the target on the obtained reconstructions by studying different approximations (Born) to speed up the computation. The recording of the phase in the optical domain is tricky, for it requires an interferometric setup. I have built a reconstruction technique based only on the intensities. I show that the obtained reconstructions are close to those obtained with the complete dataset field + phase. Finally, we propose to adjoin phaseless diffraction tomography to fluorescence structured illumination microscopy. In this framework, we illustrate the impact of the modelization of the illumination excitating the fluorophores on the resolving power of the imaging technique. Moreover, we demonstrate an imaging method which estimates simultaneously the fluorophores and the surrounding media from the joint recording of diffraction and fluorescence intensities.
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Submitted on : Tuesday, May 26, 2020 - 11:37:38 AM
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Kevin Unger. Tomographie de diffraction sans mesure de phase en régime de diffusion multiple. Optique [physics.optics]. Aix Marseille Université, Ecole doctorale N°352 : Physique et Sciences de la Matière, 2019. Français. ⟨tel-02624407⟩

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