, Filtering of data: how and how much should our data be filtered to eliminate false positives that would bias our counting. -Application of the ? c counting strategy: what is the best way to obtain a ? c curve (from experimental or simulated data), and what is the range of stoichiometries that can be determined?
, There are yet many obstacles to overcome until we can perform reliable molecular counting using FPs. However, the field will greatly benefit from new studies applying previously developed approaches. This collective experience, as much as cutting-edge developments, will contribute to eventually making qPALM a standard
, Update parameters" is pressed). The length of the phase (i.e. number of cycles performed) is written under "Time (frames), The resulting text file is organized in "phases", which consist of consecutive cycles without changes in parameters (a new phase is created each time
, You can edit all parameters in the file manually for later use with auto-control (see next section)
, However, since we are running a french version of Labview, remember to use commas (",") as decimal separators, since points will not be recognized as such. You can also suppress phases
, Using auto-control
, Auto-control allows the software to read and execute a sequence of laser patterns from a text file (in the same format as produced by Recording)
, If you edit and save the text file, the changes should be immediately visible in Labview. To start the experiment, press "Run". Phase number and duration are displayed in the auto-control panel, as well as a progression bar for the current phase. Note that if you are running in auto-control mode, you cannot manually modify lasers or EMCCD parameters
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, son attitude toujours positive et ses remarques toujours pertinentes ; c'est grâceà toi que durant ces trois ans j'ai pu prendre plaisirà découvrir la photophysique, età me creuser la tête sur toutes les questions passionnantes que l'on a pu aborder. Tu m'as apprisà regarder derrière les questions apparemment insolubles, et a toujours chercher les détails, jusqu'au "clic" de compréhension. Merci ! Un grand merci aussià l'équipe Pixel : Virgile, Joël, Sasha, Martin, Ninon, sans qui mes journées n'auraient pasété les mêmes
, This thesis would also not have been the same without our scientific collaborators, so many thanks to Elke De Zitter, Viola Mönkenmöller, Siewert Hugelier and Peter Dedecker, for your patience, and for all the work done
, I'm really proud of the work we've achieved. Vielen dank auch zu Jörg Enderlein, wer mir zweimal in Göttingen wilkommen hat; es war immer ein Vergnügen und wirklich interessant
, La recherche ne fait pas tout dans une thèse, et j'aiété très heureux de pouvoirégalement enseigner ; et pour a je voudrais vraiment remercier Claire Durmort, qui m'en a donné la possibilité, et Pierre Cavailles, Véronique Frachet et Cécile Batandier pour leur aide
Chauvet de m'avoir permis de venir parlerà sesélèves le temps d'un cours, ca aété une expérience vraiment nouvelle et enrichissante ,
, Rien n'aurait non plusété pareil si je n'avais pas rencontré ici des gens fantastiques avec qui parler de plein de choses le soir autour d'une bière ou d'un pho
, Et enfin, mercià Lisa pour ton soutien, and cheers to new adventures coming soon! Chapeau !