Skip to Main content Skip to Navigation
Theses

Study of the co-translational assembly mechanism of transcription complexes in mammalian cells

Abstract : Majority of the biological processes are carried out by multisubunit protein complexes in cells and a significant amount of energy is required by the cells to build these huge complexes. Unlike bacteria, genes encoding proteins are dispersed in the genome of eukaryotes and this makes the assembly of protein complexes more complicated to understand. By using RNA immunoprecipitation followed by genome-wide detection of mRNAs by microarray analysis, single molecule RNA FISH, immunofluoresence, mouse knock-out embryonic stem cells and domain swapping approaches, we show that the mammalian multisubunit transcription complexes assemble co-translationally. We demonstrate that the dimerization domains and their positions in the interacting subunits determine the co-translational assembly pathway (simultaneous or sequential). Furthermore, cytoplasmic IF-smFISH and two-colour smFISH experiments indicate that the described co-translational assembly is clearly occurring in the cytoplasm of human cells. Identical results in yeast, mouse and human cells suggests that co-translational assembly is a general mechanism in eukaryotes which might be necessary to avoid non-specific interactions and protein aggregation in the cell.
Complete list of metadatas

Cited literature [583 references]  Display  Hide  Download

https://tel.archives-ouvertes.fr/tel-02550614
Contributor : Abes Star :  Contact
Submitted on : Wednesday, April 22, 2020 - 12:43:07 PM
Last modification on : Thursday, June 4, 2020 - 5:45:27 PM

File

Mukherjee_Pooja_2019_ED414.pdf
Version validated by the jury (STAR)

Identifiers

  • HAL Id : tel-02550614, version 1

Collections

Citation

Pooja Mukherjee. Study of the co-translational assembly mechanism of transcription complexes in mammalian cells. Genomics [q-bio.GN]. Université de Strasbourg, 2019. English. ⟨NNT : 2019STRAJ051⟩. ⟨tel-02550614⟩

Share

Metrics

Record views

85

Files downloads

78