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Étude des communautés bactériennes d’un réseau d’eau potable. Influence des paramètres environnementaux

Abstract : Bacteria are an indivisible component of drinking water distribution systems which, by colonizing them, form complex communities. It is assumed that variations in the structure and composition of this microbiome could reflect a change in water quality related to different events (stagnation, biocorrosion, pressure change, etc.). A major sampling campaign was conducted within the Paris drinking water distribution systems allowing to assess the diversity and the dynamic of these bacterial communities using three different methods: description of the community by metabarcoding, heterotrophic plate count and quantification by PCR of three opportunistic pathogens, Mycobacterium spp. L. pneumophila, and P. aeruginosa. This study revealed some diversity within the drinking water bacterial communities. Culture and quantitative PCR methods also reveal the ecological importance of the Mycobacterium genus. The integration of spatio-temporal and physico-chemical parameters of drinking water into analyses has highlighted a relative stability of bacterial communities. However, variations in the structure of the communities are visible following important climatic events, without consequences on the sanitary quality of the water. Altogether, our work suggests that the use in parallel of different methodologies, particularly metabarcoding, has improved our knowledge of the bacterial communities within the Paris drinking water distribution systems and better observe subtle changes, invisible to conventional methods.
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Yoann Perrin. Étude des communautés bactériennes d’un réseau d’eau potable. Influence des paramètres environnementaux. Sciences agricoles. Université de Poitiers; Eau de Paris, 2018. Français. ⟨NNT : 2018POIT2289⟩. ⟨tel-02536403⟩

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