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Apport de la transgénèse murine dans l’étude des mucines gélifiantes : intérêt dans la compréhension de la physiologie de l’épithélium nasal et la physiopathologie de l’atteinte intestinale consécutive à une chimiothérapie

Abstract : Mucus is a complex viscoelastic gel. It is essential for maintaining the mucosa integrity and their functions. Its organic fraction is mainly represented by gel forming mucins (GFM). Only animal models genetically modified by recombination allow the study of their roles under physiological or pathological conditions in a perfectly an integrated system.We used this type of transgenic mouse model: 1- to specify the expression and localization of the two main nasal cavity GFM, Muc5ac and Muc5b, from Muc5b-GFP and Muc5b -/- mice; 2- to study the consequences of induction chemotherapy on the ileal intestinal epithelium and to evaluate the benefit of a strengthen mucus from Tg222 mice in this context.Our results show: 1- under physiological conditions, Muc5ac which is preferentially fucosylated, is produced in the respiratory epithelium, whereas Muc5b, which is preferentially sialylated, is produced by the Bowman glands in the olfactory epithelium; 2- after chemotherapy, a strengthen mucus does not reduce the initial damage of the ileal epithelium which are aggravated by the presence of the microbiota, but then it is the interaction between this microbiota and mucus that allows better regeneration of this epithelium.These results highlight the essential role mucus plays in the interaction between the microbiota and the mucosal epithelium. They offer interesting perspectives of research in the understanding of the impairment of olfaction and intestinal damage in patients receiving chemotherapy.
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Salah Eddine Amini. Apport de la transgénèse murine dans l’étude des mucines gélifiantes : intérêt dans la compréhension de la physiologie de l’épithélium nasal et la physiopathologie de l’atteinte intestinale consécutive à une chimiothérapie. Médecine humaine et pathologie. Université du Droit et de la Santé - Lille II, 2019. Français. ⟨NNT : 2019LIL2S032⟩. ⟨tel-02515947⟩

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