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Dynamic architectures of in vitro neuronal networks

Abstract : The function of the nervous system relies on the establishment of complex neuronal circuitry. During development, axon branching allows each neuron to establish synaptic contacts with multiple targets and is essential to the assembly of highly interconnected networks. Therefore, understanding the mechanisms underlying the control of neuronal branching is crucial in the study of neuronal circuit development.In this thesis, we investigated this phenomenon by imposing morphological constraints to neurons through the use of different chemical micropatterning techniques. Using static micropatterns, we explored branching behavior in a wide range of geometries with a focus on the influence of branching angle. In parallel, we have also worked on the development of a dynamic patterning technique based on spontaneous adsorption of comb-like derivatives of poly-L-lysine to form switchable patterns on highly cell-repellent surfaces, with the aim of creating a platform allowing for spatio-temporally controlled generation of neurite branches.
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  • HAL Id : tel-02512337, version 1


Floriane Cohen. Dynamic architectures of in vitro neuronal networks. Neurons and Cognition [q-bio.NC]. Université Paris sciences et lettres, 2018. English. ⟨NNT : 2018PSLET036⟩. ⟨tel-02512337⟩



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