Skip to Main content Skip to Navigation
Theses

Identification fine des cellules plasmocytaires normales et tumorales dans la moelle osseuse de patients atteints de myélome multiple en cytométrie en flux

Abstract : Multiple myeloma (MM) is a hematological malignancy characterized by clonal plasma cell proliferation in bone marrow and abnormal monoclonal immunoglobulin accumulation in the serum and/or urine. The heterogeneity of the disease is partly due to the cytogenetic abnormalities diversity making the disease more or less aggressive. MM is incurable in the majority of cases with a median survival between 5 and 7 years. The persistence of abnormal plasma cells in bone marrow after treatment is called minimal residual disease (MRD) and leads to the patient relapse. Multiparametric flow cytometry (MFC) is a sensitive, simple and fast technique to identify and characterize cells of interest in biological samples. In order to simplify MRD follow-up we have developed a complete solution based on MFC. This solution includes (i) the 5-color panel design, composed of anti-CD38, anti-kappa and anti-lambda antibodies (Ab) to identify total plasma cell population and two pools of Ab paired to the same fluorophore (anti-CD19/anti-CD27, negative pool and anti-CD56/anti-CD117/anti-CD200, positive pool) to detect abnormal plasma cells; (ii) the development of a device used to automatically prepare biological samples before MFC; and (iii) the analysis automation of MFC results using a homemade software. This fully automated solution increases reproducibility and productivity, decreases processing and analyzing time as well as test cost, without affecting sensibility and specificity. In parallel, we have built a simple risk score based on gene expression encoding surface proteins (CD24, CD27, CD36 and CD302) providing MM patient outcome at diagnostic and MRD follow-up.
Document type :
Theses
Complete list of metadatas

Cited literature [380 references]  Display  Hide  Download

https://tel.archives-ouvertes.fr/tel-02502295
Contributor : Abes Star :  Contact
Submitted on : Monday, March 9, 2020 - 10:28:08 AM
Last modification on : Tuesday, September 8, 2020 - 4:02:26 AM
Long-term archiving on: : Wednesday, June 10, 2020 - 1:48:58 PM

File

2017_ALATERRE_archivage.pdf
Version validated by the jury (STAR)

Identifiers

  • HAL Id : tel-02502295, version 1

Collections

Citation

Elina Alaterre. Identification fine des cellules plasmocytaires normales et tumorales dans la moelle osseuse de patients atteints de myélome multiple en cytométrie en flux. Médecine humaine et pathologie. Université Montpellier, 2017. Français. ⟨NNT : 2017MONTT010⟩. ⟨tel-02502295⟩

Share

Metrics

Record views

115

Files downloads

702