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, Evolution en fonction de la maturité de la culture
, Efficacité du transport mucociliaire en fonction de la densité de battement, p.228
, Coordination spatio-temporelle du battement ciliaire
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, Matériels et produits dont il faut disposer absolument avant de commencer : Matériels : -Bain avec ondes ultrasoniques (BRANSON 2510) -Flux de Plasma (Harrick Plasma, PLASMA CLEANER PDC-002-CE et PLASMAFLO PDC-FMG)
, Hotte à extraction vers l'extérieur (attention l'une des étapes nécessite une hotte car les produits utilisés ne doivent pas être respirés !) -Four Produits : -P-Xylène (SIGMA-ALDRICH, 95682 ou 926333) liquide -(3-glycidoxypropyl)trimethoxysilane (SIGMA-ALDRICH, 440167) appelé Glymo -Acétone -PolyEthylène-Glycol appelé PEG
, Le traitement du verre au PEG peut se décomposer en trois grandes étapes : le nettoyage du verre pour le débarrasser de toutes impuretés, un premier traitement pour charger la surface du verre et permettre au PEG d'y adhérer par la suite
, Etape 1 : Nettoyage du verre 1-Découper les lames à la dimension souhaitée avec une pointe de diamant 2-Faire une marque avec une pointe de diamant sur la face à traiter, Nettoyer les lames et lamelles dans bain d'eau distillée avec ultrason. 4-Sécher avec un pistolet à Azote
, Etape 2 : Traitement de la surface du verre en vue de l'application du PEG 5-Passage au Plasma sur les deux faces des lamelles et sur la face à traiter des lames. Application d'un flux d'oxygène pendant 5 min à une pression d'environ 500 MTorr. Cela permet de nettoyer tous les dépôts organiques sur le verre et de créer des silanes (SiH4) à sa surface qui
, Pirex) une grille afin de faciliter la récupération des lames et lamelles en suite. Sous une hotte verser bande de solution de Glymo / Xylène (P-Xylène) (1/9). La petite bande de solution déposée doit être suffisante pour que lorsqu'on dépose la lamelle, la face inférieure soit totalement recouverte de la solution
, Laver sous la hotte l'excès de Glymo avec de l'acétone. Préparer un récipient d'acétone et plonger les lames et lamelles dedans (idéalement à la vertical), Sécher les lames et lamelles
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